※ No density gradient
centrifugation, no erythrocyte lysis
※ Automation for standardization
and ease of use
※ Excellent purities and
recoveries from every sample
MACS Whole Blood MicroBeads allow magnetic cell sorting directly from whole blood or bone marrow. Leukocyte subsets are purified by using the autoMACS Pro Separator or the Whole Blood Column Kit with the MultiMACS Cell24 Separator Plus or manual separators. Pre-enrichment of white blood cells, e.g., by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analysis, molecular biology studies, such as lineage-specific chimerism analysis, and functional studies.
Basic principle
Whole
Blood MicroBeads were developed for rapid positive selection of cell subsets
directly from whole blood or bone marrow. No sample preparation is required,
including density gradient centrifugation or erythrocyte lysis. Anticoagulated
samples of 0.25–15 mL are labeled with Whole Blood MicroBeads to be
subsequently separated with the autoMACS Pro Separator or using the Whole Blood
Column Kit.
Automated cell isolation
Manual cell isolation
The
Whole Blood Column Kit is suited for the separation of leukocyte subsets
directly from anticoagulated human whole blood or bone marrow. The kit contains
Whole Blood Columns and Whole Blood Column Elution Buffer for convenient
positive selection of cells labeled with Whole Blood MicroBeads.
Use
up to 9 Whole Blood Columns on the MultiMACS Cell24 Separator Plus in
combination with the Single-Column Adapter to achieve the most convenient cell
isolation directly from multiple whole blood samples.